Syllabus
Andhra University Post Graduation Program, M.Sc Microbiology (Final) Industrial Microbiology & Medical - Practicals Syllabus
Periods/week: 8
Duration of Examination: 6 Hours
Max. Marks: 100
1. Fermentative production of ethyl alcohol by Yeast.
2. Production of Citric acid by Aspergillus niger.
3. Microbiological assay of Vitamin B12, amino acid - Lysine/Glutamic acid, antibiotic (Streptomycin)
4. Production of protease/glucose isomerase by batch fermentation.
5. Enzyme and whole cell immobilization.
6. Preparation of fermented product - Yogurt from milk.
7. Preparation of Biofertilizer and Biopesticides.
8. Strain improvement using a chemical mutagens.
9. Production, extraction and purification of any antibiotic.
10. Preparation of different media used in diagnostic microbiology.
11. Laboratory examination of sputum: Collection of sputum. Microbiological examination of sputum for pus cells and predominant bacteria. Ziehl-Neelsen staining to detect AFB culturing the specimen.
12. Collection of throat swabs - culturing the specimen.
13. Laboratory examination of pus and skin specimens for Staphylococcus aurcus, Streptococcus pyogenes and Pseudomonas aeruginosa.
14. Examinations of urine for pathogenic microorganisms - collection of urine, microscopic examination of Urine, comparison of normal specimen with unitary track infection sample - The Enterobacteriaceae - Escherichia coli, Klebsiella aerogenes and Proteus mirabilis. Urine cultures, single colonies, seeding in peptone water and Christensen’s urea medium. Examination of blood agar nutrient agar and Mac COA plate cultures
15. Mycology - Laboratory diagnosis of fungal diseases. Direct microscopy - cultures using Sabouraud’s Dextrose Agar medium - Fungi pathogenic for humans - Filamentous fungi, yeasts, yeast-like fungi, and dimorphic fungi. Aspergillus niger, Nocardia, Candida albicans.
16. Medical Parasitology - E. histolytica, G. laniblia, Trypanaosinas, Leishmania and Plasmodium.
17. Laboratory diagnosis of common helminth infections.