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Syllabus


 

Andhra University Post Graduation Program, M.Sc Microbiology (Final) Microbial Genetics & Immunology - Practicals Syllabus

Periods/week: 8
Duration of Examination: 6 Hours
Max. Marks: 100

1. Isolation of DNA (from bacterial)

2. Isolation of RNA (yeast)

3. Isolation of Plasmid DNA

4. Restriction enzyme digestion - ligation of plasmid DNA

5. Trans formation

6. Bacteriophage titration - Plaque Forming Units (PFU)

7. UV survival curve of E.coli or any other bacteria.

8. Study of repair mechanism for damage caused by UV radiation

9. Induction of P-galactosidase in E coli.

10. Isolation of mutants using EMS (Ethyl Methonyl Sulfonate)

11. Polymerase Chain Reaction (PCR)

12. Demonstration of protoplast fusion

13. Interrupted mating experiments during the study of conjugation

14. Serological tests - Widal test, VDRL test, Haemagglutination test, WBC count. ion Haemoglobin estimation, RBC count, bleeding time, - Rate (ESR), Packed Cell Volume (PCV), Blood grouping. Rh factor, ELISA

15. Diagnosis of infectious diseases using Western blotting

16. Separation of serum - Immunoelectrophoresis

17. Immuno blotting

18. Ouchterlony double diffusion

19. Radial Immunodiffusion

20. Immunoprecipitation and precipitin curve

21. Pregnancy test

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